Process Samples #4387
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| name: Process Samples | |
| on: | |
| schedule: | |
| - cron: '0 0-16/3 * * *' | |
| workflow_dispatch: | |
| concurrency: | |
| group: ${{ github.repository }} | |
| env: | |
| NXF_VER: "24.10.4" | |
| NXF_WORK: ${{ github.workspace }}/work | |
| NXF_OUTPUT: ${{ github.workspace }}/outputs | |
| NXF_NAME: github-${{ github.run_number }} | |
| GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} | |
| BATCH_SIZE: 50 | |
| defaults: | |
| run: | |
| shell: bash -eli {0} | |
| jobs: | |
| run: | |
| runs-on: self-hosted | |
| permissions: | |
| contents: write | |
| id-token: write | |
| steps: | |
| - name: Checkout main | |
| uses: actions/checkout@v4 | |
| with: | |
| token: ${{ secrets.GITHUB_TOKEN }} | |
| - name: Cache envs | |
| id: cache-envs | |
| uses: actions/cache@v4 | |
| with: | |
| path: | | |
| ${{ env.NXF_WORK }}/conda | |
| $CONDA_PREFIX/envs | |
| key: env-${{ env.NXF_VER }}-${{ github.job }} | |
| restore-keys: | | |
| env-${{ env.NXF_VER }}-${{ github.job }} | |
| env-${{ env.NXF_VER }} | |
| env- | |
| - name: 'Check and setup conda environment' | |
| run: | | |
| mamba env update --name freyja-sc2 --file environment.yml --prune | |
| - id: 'auth' | |
| name: 'Authenticate with gcloud' | |
| uses: 'google-github-actions/auth@v2' | |
| with: | |
| workload_identity_provider: 'projects/12767718289/locations/global/workloadIdentityPools/github-actions/providers/freyja-sc2' | |
| service_account: '[email protected]' | |
| - name: 'Set up Cloud SDK' | |
| uses: 'google-github-actions/setup-gcloud@v2' | |
| with: | |
| version: '>= 363.0.0' | |
| - name: Run pipeline on new samples | |
| run: | | |
| mamba activate freyja-sc2 | |
| nextflow run main.nf \ | |
| --num_samples $BATCH_SIZE \ | |
| -profile local \ | |
| -entry fetch | |
| - name: Aggregate outputs | |
| run: | | |
| mamba activate freyja-sc2 | |
| python scripts/aggregate_demix.py | |
| python scripts/aggregate_variants.py | |
| python scripts/aggregate_metadata.py | |
| - id: 'download-aggregated-outputs' | |
| name: 'Download aggregated outputs' | |
| run: | | |
| gcloud storage cp gs://outbreak-ww-data/aggregate/aggregate_demix.json outputs/aggregate/aggregate_demix.json --billing-project=andersen-lab-primary | |
| gcloud storage cp gs://outbreak-ww-data/aggregate/aggregate_variants.json outputs/aggregate/aggregate_variants.json --billing-project=andersen-lab-primary | |
| gcloud storage cp gs://outbreak-ww-data/aggregate/aggregate_metadata.json outputs/aggregate/aggregate_metadata.json --billing-project=andersen-lab-primary | |
| - id: 'concatenate-outputs' | |
| name: 'Concatenate outputs' | |
| run: | | |
| mamba activate freyja-sc2 | |
| python scripts/concat_agg_files.py | |
| - id: 'create-demix-by-week' | |
| name: 'Create demix by week' | |
| run: | | |
| mamba activate freyja-sc2 | |
| python scripts/aggregate_demix_by_week.py | |
| - id: 'upload-outputs' | |
| name: 'Upload Outputs to Cloud Storage' | |
| uses: 'google-github-actions/upload-cloud-storage@v2' | |
| with: | |
| path: 'outputs/' | |
| destination: 'outbreak-ww-data/' | |
| parent: false | |
| project_id: 'andersen-lab-primary' | |
| - name: 'Update processed samples' | |
| run: | | |
| mamba activate freyja-sc2 | |
| python scripts/update_sample_status.py $BATCH_SIZE | |
| - name: 'Commit and push changes' | |
| run: | | |
| git config --local user.name "$GITHUB_ACTOR" | |
| git config --local user.email "[email protected]" | |
| git remote set-url origin https://github.com/andersen-lab/freyja-sc2 | |
| git add data/all_metadata.csv | |
| git commit -m "Update processed samples" | |
| git push --force | |
| - name: 'Clean workspace' | |
| run: | | |
| rm -rf ${{ github.workspace }}/* |